TRIO Protocol: Circadian Nutrition Science<\/title>\n <style>\n body {\n font-family: 'Arial', sans-serif;\n background-color: #f9f5f0;\n color: #333;\n line-height: 1.6;\n margin: 0;\n padding: 0;\n display: flex;\n justify-content: center;\n align-items: center;\n min-height: 100vh;\n }\n \n .container {\n max-width: 800px;\n background: white;\n border-radius: 12px;\n box-shadow: 0 10px 30px rgba(0, 0, 0, 0.1);\n padding: 30px;\n margin: 20px;\n }\n \n h1 {\n color: #8b4513;\n text-align: center;\n font-size: 28px;\n margin-bottom: 10px;\n font-weight: 700;\n }\n \n h2 {\n color: #8b4513;\n font-size: 22px;\n margin-top: 30px;\n border-bottom: 2px solid #d4a76a;\n padding-bottom: 5px;\n }\n \n h3 {\n color: #d2691e;\n font-size: 18px;\n margin-top: 25px;\n }\n \n .subtitle {\n color: #a0522d;\n text-align: center;\n font-size: 16px;\n margin-bottom: 30px;\n font-style: italic;\n }\n \n .highlight-box {\n background-color: #fffaf0;\n border-left: 4px solid #d4a76a;\n padding: 15px;\n margin: 20px 0;\n border-radius: 0 8px 8px 0;\n }\n \n table {\n width: 100%;\n border-collapse: collapse;\n margin: 20px 0;\n font-size: 15px;\n }\n \n th, td {\n padding: 12px;\n text-align: left;\n border-bottom: 1px solid #ddd;\n }\n \n th {\n background-color: #f5e7d8;\n color: #8b4513;\n }\n \n .mermaid {\n background-color: #fffaf0;\n padding: 15px;\n border-radius: 8px;\n margin: 20px 0;\n overflow-x: auto;\n }\n \n .important {\n color: #d2691e;\n font-weight: 600;\n }\n \n .citation {\n font-size: 13px;\n color: #a0522d;\n font-style: italic;\n }\n \n .footer {\n text-align: center;\n margin-top: 40px;\n color: #a0522d;\n font-size: 14px;\n border-top: 1px solid #d4a76a;\n padding-top: 20px;\n }\n <\/style>\n<\/head>\n<body>\n <div class=\"container\">\n <h1>The TRIO Protocol: Circadian-Aligned Nutritional Framework<\/h1>\n <p class=\"subtitle\">Evidence-Based Synchronization of Nutrient Timing with Cellular Physiology<\/p>\n \n <div class=\"highlight-box\">\n A <span class=\"important\">time-targeted<\/span> nutritional system aligning amino acid availability and energy substrate utilization with <span class=\"important\">circadian metabolic cycling<\/span> (Panda, 2016 <em>Cell Metab<\/em>).\n <\/div>\n \n <h2>1. Biological Foundations<\/h2>\n <p>The human organism functions as a dynamic biochemical network dependent on:<\/p>\n <ul>\n <li><span class=\"important\">Amino acids<\/span> (structural\/functional components)<\/li>\n <li><span class=\"important\">Energy substrates<\/span> (ATP precursors: glucose, fatty acids, ketones)<\/li>\n <\/ul>\n \n <h3>1.1 Diurnal Metabolic Cycling<\/h3>\n <table>\n <tr>\n <th>Phase<\/th>\n <th>Dominant Pathway<\/th>\n <th>Primary Fuel<\/th>\n <th>Metabolic State<\/th>\n <\/tr>\n <tr>\n <td><strong>Daytime (Active)<\/strong><\/td>\n <td>Glycolysis\/Oxidative Phosphorylation<\/td>\n <td>Glucose (\u2192ATP)<\/td>\n <td>Catabolic (energy utilization)<\/td>\n <\/tr>\n <tr>\n <td><strong>Nighttime (Rest)<\/strong><\/td>\n <td>Protein Synthesis\/Autophagy<\/td>\n <td>Amino Acids<\/td>\n <td>Anabolic (tissue repair)<\/td>\n <\/tr>\n <\/table>\n <p class=\"citation\">Circadian regulation of human metabolism (Huang et al., 2021 <em>Nature Reviews Endocrinology<\/em>)<\/p>\n \n <h2>2. Daytime Physiology<\/h2>\n <h3>2.1 Glucose Metabolism<\/h3>\n <ul>\n <li><span class=\"important\">GLUT4 transporters<\/span> mediate insulin-dependent glucose uptake in myocytes<\/li>\n <li><span class=\"important\">Pyruvate dehydrogenase<\/span> converts glucose \u2192 Acetyl-CoA for TCA cycle<\/li>\n <\/ul>\n \n <h3>2.2 Stimulus-Response Pathways<\/h3>\n <p>External stimuli activate:<\/p>\n <ul>\n <li><span class=\"important\">MAPK\/NF-\u03baB signaling<\/span> \u2192 Transcriptional adaptation<\/li>\n <li><span class=\"important\">mTORC1 activation<\/span> \u2192 Ribosomal biogenesis<\/li>\n <\/ul>\n \n <div class=\"highlight-box\">\n <strong>TRIO Prescription (06:00\u201314:00):<\/strong><br>\n \u2022 Low-protein, high-phytonutrient foods (berries, leafy greens)<br>\n \u2022 Carbohydrate focus: Fructose \u2192 hepatic glycogen replenishment\n <\/div>\n \n <h2>3. Nighttime Physiology<\/h2>\n <h3>3.1 Ribosomal Translation<\/h3>\n <ul>\n <li><span class=\"important\">eIF4F complex<\/span> mediates cap-dependent translation<\/li>\n <li><span class=\"important\">Collagen synthesis<\/span> peaks during NREM sleep<\/li>\n <\/ul>\n \n <h3>3.2 Ruminant Nutrient Advantages<\/h3>\n <p>Beef\/lamb provide:<\/p>\n <ul>\n <li>Complete EAA profile (leucine \u2192 mTOR activation)<\/li>\n <li>Heme iron (optimal bioavailability)<\/li>\n <li>Conjugated linoleic acid (CLA) \u2192 PPAR-\u03b3 activation<\/li>\n <\/ul>\n \n <div class=\"highlight-box\">\n <strong>TRIO Prescription (14:00\u201318:00):<\/strong><br>\n \u2022 Ruminant meats + organ meats (liver for retinol\/B12)<br>\n \u2022 Digestive support: Betaine HCl (gastric pH \u22641.5 for pepsin)\n <\/div>\n \n <h2>4. Molecular Integration<\/h2>\n <div class=\"mermaid\">\n <strong>Metabolic Pathway Map:<\/strong><br>\n [Daytime Glucose] \u2192 ATP via OXPHOS<br>\n [Evening Proteins] \u2192 Amino Acid Pool \u2192 Nighttime Translation<br>\n [Circadian Entrainment] \u2192 AMPK\/SIRT1 Synchronization\n <\/div>\n \n <h2>5. Evidence-Based Implementation<\/h2>\n <h3>5.1 Meal Timing<\/h3>\n <ul>\n <li>Last meal \u22653h before sleep: Ghrelin\/leptin reset<\/li>\n <li>14h overnight fast: Induces autophagy<\/li>\n <\/ul>\n \n <h3>5.2 Contraindications<\/h3>\n <ul>\n <li>Diabetes mellitus: Requires glucose monitoring<\/li>\n <li>Pregnancy: Increased protein needs<\/li>\n <\/ul>\n \n <div class=\"footer\">\n TRIO Protocol \u00a9 | Peer-Reviewed Citations Available Upon Request\n <\/div>\n <\/div>\n<\/body>\n<\/html>","protected":false},"excerpt":{"rendered":"<p>TRIO Protocol: Circadian Nutrition Science The TRIO Protocol: Circadian-Aligned Nutritional Framework Evidence-Based Synchronization of Nutrient Timing with Cellular Physiology A time-targeted nutritional system aligning amino acid availability and energy substrate…<\/p><a href='https://aihealthinsight.org/tag/amino-acids/'>men viagra before and after photos</a> <a href='https://aihealthinsight.org/quanto-dura-l-effetto-del-viagra/'>quanto dura l effetto del viagra</a> <a href='https://aihealthinsight.org/viagra-senza-ricetta-a-roma/'>viagra senza ricetta a roma</a> <a href='https://aihealthinsight.org/cialis-generico-prezzo-in-farmacia-italiana/'>cialis generico prezzo in farmacia italiana</a> <a href='https://aihealthinsight.org/cialis-generico-senza-ricetta-in-farmacia/'>cialis generico senza ricetta in farmacia</a>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_vp_format_video_url":"","_vp_image_focal_point":[],"footnotes":""},"categories":[1],"tags":[],"class_list":["post-1575","post","type-post","status-publish","format-standard","hentry","category-2024-upgrade"],"_links":{"self":[{"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/posts\/1575","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/comments?post=1575"}],"version-history":[{"count":1,"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/posts\/1575\/revisions"}],"predecessor-version":[{"id":1576,"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/posts\/1575\/revisions\/1576"}],"wp:attachment":[{"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/media?parent=1575"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/categories?post=1575"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/tags?post=1575"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

{"id":1575,"date":"2025-04-02T18:49:11","date_gmt":"2025-04-02T18:49:11","guid":{"rendered":"https:\/\/aihealthinsight.org\/?p=1575"},"modified":"2025-04-02T18:49:13","modified_gmt":"2025-04-02T18:49:13","slug":"the-trio-protocol-circadian-aligned-nutritional-framework","status":"publish","type":"post","link":"https:\/\/aihealthinsight.org\/the-trio-protocol-circadian-aligned-nutritional-framework\/","title":{"rendered":"The TRIO Protocol: Circadian-Aligned Nutritional Framework."},"content":{"rendered":"\n\n\n \n \n TRIO Protocol: Circadian Nutrition Science<\/title>\n <style>\n body {\n font-family: 'Arial', sans-serif;\n background-color: #f9f5f0;\n color: #333;\n line-height: 1.6;\n margin: 0;\n padding: 0;\n display: flex;\n justify-content: center;\n align-items: center;\n min-height: 100vh;\n }\n \n .container {\n max-width: 800px;\n background: white;\n border-radius: 12px;\n box-shadow: 0 10px 30px rgba(0, 0, 0, 0.1);\n padding: 30px;\n margin: 20px;\n }\n \n h1 {\n color: #8b4513;\n text-align: center;\n font-size: 28px;\n margin-bottom: 10px;\n font-weight: 700;\n }\n \n h2 {\n color: #8b4513;\n font-size: 22px;\n margin-top: 30px;\n border-bottom: 2px solid #d4a76a;\n padding-bottom: 5px;\n }\n \n h3 {\n color: #d2691e;\n font-size: 18px;\n margin-top: 25px;\n }\n \n .subtitle {\n color: #a0522d;\n text-align: center;\n font-size: 16px;\n margin-bottom: 30px;\n font-style: italic;\n }\n \n .highlight-box {\n background-color: #fffaf0;\n border-left: 4px solid #d4a76a;\n padding: 15px;\n margin: 20px 0;\n border-radius: 0 8px 8px 0;\n }\n \n table {\n width: 100%;\n border-collapse: collapse;\n margin: 20px 0;\n font-size: 15px;\n }\n \n th, td {\n padding: 12px;\n text-align: left;\n border-bottom: 1px solid #ddd;\n }\n \n th {\n background-color: #f5e7d8;\n color: #8b4513;\n }\n \n .mermaid {\n background-color: #fffaf0;\n padding: 15px;\n border-radius: 8px;\n margin: 20px 0;\n overflow-x: auto;\n }\n \n .important {\n color: #d2691e;\n font-weight: 600;\n }\n \n .citation {\n font-size: 13px;\n color: #a0522d;\n font-style: italic;\n }\n \n .footer {\n text-align: center;\n margin-top: 40px;\n color: #a0522d;\n font-size: 14px;\n border-top: 1px solid #d4a76a;\n padding-top: 20px;\n }\n <\/style>\n<\/head>\n<body>\n <div class=\"container\">\n <h1>The TRIO Protocol: Circadian-Aligned Nutritional Framework<\/h1>\n <p class=\"subtitle\">Evidence-Based Synchronization of Nutrient Timing with Cellular Physiology<\/p>\n \n <div class=\"highlight-box\">\n A <span class=\"important\">time-targeted<\/span> nutritional system aligning amino acid availability and energy substrate utilization with <span class=\"important\">circadian metabolic cycling<\/span> (Panda, 2016 <em>Cell Metab<\/em>).\n <\/div>\n \n <h2>1. Biological Foundations<\/h2>\n <p>The human organism functions as a dynamic biochemical network dependent on:<\/p>\n <ul>\n <li><span class=\"important\">Amino acids<\/span> (structural\/functional components)<\/li>\n <li><span class=\"important\">Energy substrates<\/span> (ATP precursors: glucose, fatty acids, ketones)<\/li>\n <\/ul>\n \n <h3>1.1 Diurnal Metabolic Cycling<\/h3>\n <table>\n <tr>\n <th>Phase<\/th>\n <th>Dominant Pathway<\/th>\n <th>Primary Fuel<\/th>\n <th>Metabolic State<\/th>\n <\/tr>\n <tr>\n <td><strong>Daytime (Active)<\/strong><\/td>\n <td>Glycolysis\/Oxidative Phosphorylation<\/td>\n <td>Glucose (\u2192ATP)<\/td>\n <td>Catabolic (energy utilization)<\/td>\n <\/tr>\n <tr>\n <td><strong>Nighttime (Rest)<\/strong><\/td>\n <td>Protein Synthesis\/Autophagy<\/td>\n <td>Amino Acids<\/td>\n <td>Anabolic (tissue repair)<\/td>\n <\/tr>\n <\/table>\n <p class=\"citation\">Circadian regulation of human metabolism (Huang et al., 2021 <em>Nature Reviews Endocrinology<\/em>)<\/p>\n \n <h2>2. Daytime Physiology<\/h2>\n <h3>2.1 Glucose Metabolism<\/h3>\n <ul>\n <li><span class=\"important\">GLUT4 transporters<\/span> mediate insulin-dependent glucose uptake in myocytes<\/li>\n <li><span class=\"important\">Pyruvate dehydrogenase<\/span> converts glucose \u2192 Acetyl-CoA for TCA cycle<\/li>\n <\/ul>\n \n <h3>2.2 Stimulus-Response Pathways<\/h3>\n <p>External stimuli activate:<\/p>\n <ul>\n <li><span class=\"important\">MAPK\/NF-\u03baB signaling<\/span> \u2192 Transcriptional adaptation<\/li>\n <li><span class=\"important\">mTORC1 activation<\/span> \u2192 Ribosomal biogenesis<\/li>\n <\/ul>\n \n <div class=\"highlight-box\">\n <strong>TRIO Prescription (06:00\u201314:00):<\/strong><br>\n \u2022 Low-protein, high-phytonutrient foods (berries, leafy greens)<br>\n \u2022 Carbohydrate focus: Fructose \u2192 hepatic glycogen replenishment\n <\/div>\n \n <h2>3. Nighttime Physiology<\/h2>\n <h3>3.1 Ribosomal Translation<\/h3>\n <ul>\n <li><span class=\"important\">eIF4F complex<\/span> mediates cap-dependent translation<\/li>\n <li><span class=\"important\">Collagen synthesis<\/span> peaks during NREM sleep<\/li>\n <\/ul>\n \n <h3>3.2 Ruminant Nutrient Advantages<\/h3>\n <p>Beef\/lamb provide:<\/p>\n <ul>\n <li>Complete EAA profile (leucine \u2192 mTOR activation)<\/li>\n <li>Heme iron (optimal bioavailability)<\/li>\n <li>Conjugated linoleic acid (CLA) \u2192 PPAR-\u03b3 activation<\/li>\n <\/ul>\n \n <div class=\"highlight-box\">\n <strong>TRIO Prescription (14:00\u201318:00):<\/strong><br>\n \u2022 Ruminant meats + organ meats (liver for retinol\/B12)<br>\n \u2022 Digestive support: Betaine HCl (gastric pH \u22641.5 for pepsin)\n <\/div>\n \n <h2>4. Molecular Integration<\/h2>\n <div class=\"mermaid\">\n <strong>Metabolic Pathway Map:<\/strong><br>\n [Daytime Glucose] \u2192 ATP via OXPHOS<br>\n [Evening Proteins] \u2192 Amino Acid Pool \u2192 Nighttime Translation<br>\n [Circadian Entrainment] \u2192 AMPK\/SIRT1 Synchronization\n <\/div>\n \n <h2>5. Evidence-Based Implementation<\/h2>\n <h3>5.1 Meal Timing<\/h3>\n <ul>\n <li>Last meal \u22653h before sleep: Ghrelin\/leptin reset<\/li>\n <li>14h overnight fast: Induces autophagy<\/li>\n <\/ul>\n \n <h3>5.2 Contraindications<\/h3>\n <ul>\n <li>Diabetes mellitus: Requires glucose monitoring<\/li>\n <li>Pregnancy: Increased protein needs<\/li>\n <\/ul>\n \n <div class=\"footer\">\n TRIO Protocol \u00a9 | Peer-Reviewed Citations Available Upon Request\n <\/div>\n <\/div>\n<\/body>\n<\/html>","protected":false},"excerpt":{"rendered":"<p>TRIO Protocol: Circadian Nutrition Science The TRIO Protocol: Circadian-Aligned Nutritional Framework Evidence-Based Synchronization of Nutrient Timing with Cellular Physiology A time-targeted nutritional system aligning amino acid availability and energy substrate…<\/p><a href='https://aihealthinsight.org/tag/amino-acids/'>men viagra before and after photos</a> <a href='https://aihealthinsight.org/quanto-dura-l-effetto-del-viagra/'>quanto dura l effetto del viagra</a> <a href='https://aihealthinsight.org/viagra-senza-ricetta-a-roma/'>viagra senza ricetta a roma</a> <a href='https://aihealthinsight.org/cialis-generico-prezzo-in-farmacia-italiana/'>cialis generico prezzo in farmacia italiana</a> <a href='https://aihealthinsight.org/cialis-generico-senza-ricetta-in-farmacia/'>cialis generico senza ricetta in farmacia</a>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_vp_format_video_url":"","_vp_image_focal_point":[],"footnotes":""},"categories":[1],"tags":[],"class_list":["post-1575","post","type-post","status-publish","format-standard","hentry","category-2024-upgrade"],"_links":{"self":[{"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/posts\/1575","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/comments?post=1575"}],"version-history":[{"count":1,"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/posts\/1575\/revisions"}],"predecessor-version":[{"id":1576,"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/posts\/1575\/revisions\/1576"}],"wp:attachment":[{"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/media?parent=1575"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/categories?post=1575"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/aihealthinsight.org\/wp-json\/wp\/v2\/tags?post=1575"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}